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Select Month: September 2009

Advanced Lab Techniques Help Pinpoint the Source of Outbreaks

Categories: General

These are the tools used in multiple-locus variable-number tandem-repeats analysis (MLVA), a technique that has the potential to identify subtle differences at the genetic level between closely related bacterial strains.

These are the tools used in multiple-locus variable-number tandem-repeats analysis (MLVA), a technique that has the potential to identify subtle differences at the genetic level between closely related bacterial strains.

When CDC is working on an outbreak of foodborne illness, it’s our goal to pinpoint the source as quickly and precisely as possible so we can prevent any further threat to public health. For several years now, the PulseNet Methods Development and Reference Unit (PMDRU) has been developing and validating new methods to complement pulsed-field gel electrophoresis (PFGE), which is currently the standard typing method used in outbreak detection. The method that we have found most helpful in detecting subtle differences between closely related bacterial strains is a technique known as multiple-locus variable-number tandem repeat analysis (MLVA).

Cookie Dough - Gooey, Sweet, and Seasoned with… Bacteria?!

Categories: Foodborne

3 magnetic beads about 5 microns in diameter.

The possibility that E. coli O157:H7 was a contaminant in cookie dough surprised even the most experienced microbiologists here in CDC’s Enteric Diseases Laboratory Branch. E. coli O157 is a common culprit of a severe diarrheal illness, usually caused by eating contaminated and undercooked ground beef or drinking unpasteurized apple juice. It shouldn’t have even been on the “Who’s Who” list of the top bacterial contaminants.

The Fit Between Wildlife Health and Human Health

Categories: General

Wildlife Disease Association's logo

“Wildlife Health from Land to Sea: Impacts of a Changing World.” That was the theme of the 58th annual meeting of the Wildlife Disease Association, held earlier this month. I had the pleasure of attending this conference along with several colleagues from the National Center for Zoonotic, Vector-borne, and Enteric Diseases (NCZVED).

 

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